TRR 175 The Green Hub - Central Coordinator of Acclimation in Plants

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Dissection of the plastidic unfolded protein response in Chlamydomonas reinhardtii

In this project we aim at characterizing the chloroplast UPR (cpUPR) using Chlamydomonas as a model system. For this we will first trigger a cpUPR by inducing the expression of unfolded proteins in the chloroplast and quantify the cpUPR in a time-resolved manner by MS-based proteomics, tran-scriptomics, and metabolomics. Second, we will identify mutants defective in the cpUPR by forward and reverse genetics approaches. Third, we will functionally characterize the chloroplast universal stress protein A, a high-potential integrator of chloroplast acclimation responses. Fourth, we will sys-tem-wide monitor acclimation responses of photoautotrophically grown Chlamydomonas cells to heat and cold.